Characterization of the Recombinant Copper Chaperone (CCS) for the Copper/Zinc Superoxide Dismutase (CuZnSOD) from the plant Glycine (G.) max
نویسندگان
چکیده
Estación Experimental de Aula Dei (EEAD), Consejo Superior de Investigaciones Científicas (CSIC), Avda. Montañana 1005, E-50059 Zaragoza, Spain ([email protected];[email protected];[email protected];[email protected]). Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Ciencias and Instituto de Biocomputación y Física de Sistemas Complejos (BIFI), Universidad de Zaragoza, C/ Pedro Cerbuna 12, E-50009 Zaragoza, Spain ([email protected]). Current address: LS Pflanzenphysiologie, Ruhr-Universität Bochum, ND Nord 3, Rm 3/71-73, Universitätsstraße 150, 44801 Bochum, Germany ([email protected]).
منابع مشابه
A copper chaperone for superoxide dismutase that confers three types of copper/zinc superoxide dismutase activity in Arabidopsis.
The copper chaperone for superoxide dismutase (CCS) has been identified as a key factor integrating copper into copper/zinc superoxide dismutase (CuZnSOD) in yeast (Saccharomyces cerevisiae) and mammals. In Arabidopsis (Arabidopsis thaliana), only one putative CCS gene (AtCCS, At1g12520) has been identified. The predicted AtCCS polypeptide contains three distinct domains: a central domain, flan...
متن کاملLoss of in vitro metal ion binding specificity in mutant copper-zinc superoxide dismutases associated with familial amyotrophic lateral sclerosis.
The presence of the copper ion at the active site of human wild type copper-zinc superoxide dismutase (CuZnSOD) is essential to its ability to catalyze the disproportionation of superoxide into dioxygen and hydrogen peroxide. Wild type CuZnSOD and several of the mutants associated with familial amyotrophic lateral sclerosis (FALS) (Ala(4) --> Val, Gly(93) --> Ala, and Leu(38) --> Val) were expr...
متن کاملIsolation of a gene encoding a copper chaperone for the copper/zinc superoxide dismutase and characterization of its promoter in potato.
Gene expression during the potato (Solanum tuberosum) tuber lifecycle was monitored by cDNA-amplified fragment-length polymorphism, and several differentially expressed transcript-derived fragments were isolated. One fragment, named TDFL431, showed high homology to a copper (Cu) chaperone for Cu/zinc superoxide dismutase (CCS). The Ccs protein is responsible for the delivery of Cu to the Cu/zin...
متن کاملBinding of miR398 to mRNA of Chaperone and Superoxide Dismutase Genes in Plants
Among all microRNAs (miRNAs) in 12 plant species investigated in this study, only miR398 targeted the copper chaperone for superoxide dismutase (CCS). The nucleotide sequences of miRNA binding sites were located in the mRNA protein-coding sequence (CDS) and were highly homologous. These binding sites in CCS mRNA encoded a conservative GDLGTL hexapeptide. The binding sites for miR398 in the CDS ...
متن کاملEnhancement of Solubility and Specific Activity of a Cu/Zn Superoxide Dismutase by Co-expression with a Copper Chaperone in Escherichia coli
Background: Human Cu/Zn superoxide dismutase (hSOD1) is an antioxidant enzyme with potential as a therapeutic agent. However, heterologous expression of hSOD1 has remained an issue due to Cu2+ insufficiency at protein active site, leading to low solubility and enzymatic activity.Objectives:The effect of co-expressed human copper chaperone (hCCS) to enhance the solubility and enzymatic act...
متن کامل